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tgf-b cytokine  (PeproTech)


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    PeproTech tgf-b cytokine
    Tgf B Cytokine, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tgf-b+cytokine/tgf+b+cytokine/pmc12102334-254-0-1
    Average 90 stars, based on 1 article reviews
    tgf-b cytokine - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Positive Control:

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL .

    Article Title: GDF11 downregulates FOXP3 in T-cell acute lymphoblastic leukemia-derived cells and associates with restraining aggressiveness
    Article Snippet: Background: Growth differentiation factor 11 (GDF11), a transforming growth factor-beta superfamily member, is a crucial protein involved in many differentiation processes in embryogenesis and morphogenesis, and it has been extensively characterized due to its capacity to target poorly differentiated cells, including transformed or cancer cells.. Aim: In the present work, we aimed to describe the effects on migration, proliferation, and metabolism in the T-cell acute lymphoblastic leukemia-derived cell line Jurkat.. Methods: Based on previous evidence, we analyzed metabolic changes exerted by GDF11 and its relationship with the aggressive phenotype.

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: Cells were incubated for 72 h either without stimulation or with 1 mg/mL immobilized anti-CD3 mAb (eBioscience), 1 mg/mL soluble anti-CD28 mAb (eBioscience), 10 ng/mL IL-2 (PeproTech, London, UK), and 5 ng/mL TGF-b (PeproTech).

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis.
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL92.

    Article Title: Mechanical microscopy of cancer cells: TGF-β induced epithelial to mesenchymal transition corresponds to low intracellular viscosity in cancer cells.
    Article Snippet: Viscosity is an essential parameter that regulates bio-molecular reaction rates of diffusion-driven cellular processes.. Hence, abnormal viscosity levels are often associated with various diseases and malfunctions like cancer.. For this reason, monitoring intracellular viscosity becomes vital.

    Article Title: Mitochondrial reactive oxygen species regulate acetyl-CoA flux between cytokine production and fatty acid synthesis in effector T cells.
    Article Snippet: CD4+CD25T cells were cultured without any polarizing cytokines or antibodies for non-polarizing (ThN) conditions, with 10 mg/ml anti-IL-4 antibody (Peprotech) and 2 ng/ml IL-12 for Th1 differentiation; 20 mg/ml anti-IFN-g antibody (Bio XCell), 2.5 mg/ml anti-IL-12 antibody and 10 ng/ml IL-4 for Th2 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech) and 1 ng/ml TGF-b (Peprotech) for non-pathogenic Th17 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech), 10 ng/ml IL-23 (R&D Systems) and 10 ng/ml IL-1b (R&D Systems) for pathogenic Th17 differentiation and cultured in T cell medium as described above.

    Article Title: Multiplexed PLGA scaffolds with nitric oxide-releasing zinc oxide and melatonin-modulated extracellular vesicles for severe chronic kidney disease.
    Article Snippet: After 24 h of seeding, the cells were damaged with TGF-b (2 ng/ml, 100–21, Peprotech, NJ, USA) for 2 h and PLGA, PME, PMEZ, and PMEZ/mEV (1 108 EVs/mL) scaffolds were treated using trans-well inserts system after 24 h. Cell nucleus and fibronectin were stained using Hoechst (1 lg/ml, 62249, Thermo Fisher Scientific, OH, USA) and fibronectin primary antibody (Abcam, MA, USA) for ICC, and proteins were extracted to compare inhibition activity of scaffolds for fibrosis markers, fibronectin, using Western blot analysis.

    Article Title: Renal IL-23-Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with Kidney Fibrosis.
    Article Snippet: RESEARCH ARTICLE | JULY 29 2024 Renal IL-23–Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal In ammasome Activation with Kidney Fibrosis Teresa M. Frasconi; ... et. al J Immunol (2024) 213 (6): 865–875. https://doi.org/10.4049/jimmunol.2400041 Related Content IL-12 De ciency in MRL-Fas lpr Mice Delays Nephritis and Intrarenal IFN-γ Expression, and Diminishes Systemic Pathology J Immunol (April,2003) Ischemia Reperfusion Induces IFN Regulatory Factor 4 in Renal Dendritic Cells, which Suppresses Postischemic In ammation and Prevents Acute Renal Failure J Immunol (August,2010) Immunomodulatory Molecule IRAK-M Balances Macrophage Polarization and Determines Macrophage Responses during Renal Fibrosis J Immunol (August,2017) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/213/6/865/1661067/ji2400041.pdf by guest on 08 February 2025 Renal IL-23 Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with

    Expressing:

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL .

    Article Title: GDF11 downregulates FOXP3 in T-cell acute lymphoblastic leukemia-derived cells and associates with restraining aggressiveness
    Article Snippet: Background: Growth differentiation factor 11 (GDF11), a transforming growth factor-beta superfamily member, is a crucial protein involved in many differentiation processes in embryogenesis and morphogenesis, and it has been extensively characterized due to its capacity to target poorly differentiated cells, including transformed or cancer cells.. Aim: In the present work, we aimed to describe the effects on migration, proliferation, and metabolism in the T-cell acute lymphoblastic leukemia-derived cell line Jurkat.. Methods: Based on previous evidence, we analyzed metabolic changes exerted by GDF11 and its relationship with the aggressive phenotype.

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: Cells were incubated for 72 h either without stimulation or with 1 mg/mL immobilized anti-CD3 mAb (eBioscience), 1 mg/mL soluble anti-CD28 mAb (eBioscience), 10 ng/mL IL-2 (PeproTech, London, UK), and 5 ng/mL TGF-b (PeproTech).

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis.
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL92.

    Article Title: Mechanical microscopy of cancer cells: TGF-β induced epithelial to mesenchymal transition corresponds to low intracellular viscosity in cancer cells.
    Article Snippet: Viscosity is an essential parameter that regulates bio-molecular reaction rates of diffusion-driven cellular processes.. Hence, abnormal viscosity levels are often associated with various diseases and malfunctions like cancer.. For this reason, monitoring intracellular viscosity becomes vital.

    Article Title: Mitochondrial reactive oxygen species regulate acetyl-CoA flux between cytokine production and fatty acid synthesis in effector T cells.
    Article Snippet: CD4+CD25T cells were cultured without any polarizing cytokines or antibodies for non-polarizing (ThN) conditions, with 10 mg/ml anti-IL-4 antibody (Peprotech) and 2 ng/ml IL-12 for Th1 differentiation; 20 mg/ml anti-IFN-g antibody (Bio XCell), 2.5 mg/ml anti-IL-12 antibody and 10 ng/ml IL-4 for Th2 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech) and 1 ng/ml TGF-b (Peprotech) for non-pathogenic Th17 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech), 10 ng/ml IL-23 (R&D Systems) and 10 ng/ml IL-1b (R&D Systems) for pathogenic Th17 differentiation and cultured in T cell medium as described above.

    Article Title: Multiplexed PLGA scaffolds with nitric oxide-releasing zinc oxide and melatonin-modulated extracellular vesicles for severe chronic kidney disease.
    Article Snippet: After 24 h of seeding, the cells were damaged with TGF-b (2 ng/ml, 100–21, Peprotech, NJ, USA) for 2 h and PLGA, PME, PMEZ, and PMEZ/mEV (1 108 EVs/mL) scaffolds were treated using trans-well inserts system after 24 h. Cell nucleus and fibronectin were stained using Hoechst (1 lg/ml, 62249, Thermo Fisher Scientific, OH, USA) and fibronectin primary antibody (Abcam, MA, USA) for ICC, and proteins were extracted to compare inhibition activity of scaffolds for fibrosis markers, fibronectin, using Western blot analysis.

    Article Title: Renal IL-23-Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with Kidney Fibrosis.
    Article Snippet: RESEARCH ARTICLE | JULY 29 2024 Renal IL-23–Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal In ammasome Activation with Kidney Fibrosis Teresa M. Frasconi; ... et. al J Immunol (2024) 213 (6): 865–875. https://doi.org/10.4049/jimmunol.2400041 Related Content IL-12 De ciency in MRL-Fas lpr Mice Delays Nephritis and Intrarenal IFN-γ Expression, and Diminishes Systemic Pathology J Immunol (April,2003) Ischemia Reperfusion Induces IFN Regulatory Factor 4 in Renal Dendritic Cells, which Suppresses Postischemic In ammation and Prevents Acute Renal Failure J Immunol (August,2010) Immunomodulatory Molecule IRAK-M Balances Macrophage Polarization and Determines Macrophage Responses during Renal Fibrosis J Immunol (August,2017) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/213/6/865/1661067/ji2400041.pdf by guest on 08 February 2025 Renal IL-23 Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with

    Staining:

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL .

    Article Title: GDF11 downregulates FOXP3 in T-cell acute lymphoblastic leukemia-derived cells and associates with restraining aggressiveness
    Article Snippet: Background: Growth differentiation factor 11 (GDF11), a transforming growth factor-beta superfamily member, is a crucial protein involved in many differentiation processes in embryogenesis and morphogenesis, and it has been extensively characterized due to its capacity to target poorly differentiated cells, including transformed or cancer cells.. Aim: In the present work, we aimed to describe the effects on migration, proliferation, and metabolism in the T-cell acute lymphoblastic leukemia-derived cell line Jurkat.. Methods: Based on previous evidence, we analyzed metabolic changes exerted by GDF11 and its relationship with the aggressive phenotype.

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: Cells were incubated for 72 h either without stimulation or with 1 mg/mL immobilized anti-CD3 mAb (eBioscience), 1 mg/mL soluble anti-CD28 mAb (eBioscience), 10 ng/mL IL-2 (PeproTech, London, UK), and 5 ng/mL TGF-b (PeproTech).

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis.
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL92.

    Article Title: Mechanical microscopy of cancer cells: TGF-β induced epithelial to mesenchymal transition corresponds to low intracellular viscosity in cancer cells.
    Article Snippet: Viscosity is an essential parameter that regulates bio-molecular reaction rates of diffusion-driven cellular processes.. Hence, abnormal viscosity levels are often associated with various diseases and malfunctions like cancer.. For this reason, monitoring intracellular viscosity becomes vital.

    Article Title: Mitochondrial reactive oxygen species regulate acetyl-CoA flux between cytokine production and fatty acid synthesis in effector T cells.
    Article Snippet: CD4+CD25T cells were cultured without any polarizing cytokines or antibodies for non-polarizing (ThN) conditions, with 10 mg/ml anti-IL-4 antibody (Peprotech) and 2 ng/ml IL-12 for Th1 differentiation; 20 mg/ml anti-IFN-g antibody (Bio XCell), 2.5 mg/ml anti-IL-12 antibody and 10 ng/ml IL-4 for Th2 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech) and 1 ng/ml TGF-b (Peprotech) for non-pathogenic Th17 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech), 10 ng/ml IL-23 (R&D Systems) and 10 ng/ml IL-1b (R&D Systems) for pathogenic Th17 differentiation and cultured in T cell medium as described above.

    Article Title: Multiplexed PLGA scaffolds with nitric oxide-releasing zinc oxide and melatonin-modulated extracellular vesicles for severe chronic kidney disease.
    Article Snippet: After 24 h of seeding, the cells were damaged with TGF-b (2 ng/ml, 100–21, Peprotech, NJ, USA) for 2 h and PLGA, PME, PMEZ, and PMEZ/mEV (1 108 EVs/mL) scaffolds were treated using trans-well inserts system after 24 h. Cell nucleus and fibronectin were stained using Hoechst (1 lg/ml, 62249, Thermo Fisher Scientific, OH, USA) and fibronectin primary antibody (Abcam, MA, USA) for ICC, and proteins were extracted to compare inhibition activity of scaffolds for fibrosis markers, fibronectin, using Western blot analysis.

    Article Title: Renal IL-23-Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with Kidney Fibrosis.
    Article Snippet: RESEARCH ARTICLE | JULY 29 2024 Renal IL-23–Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal In ammasome Activation with Kidney Fibrosis Teresa M. Frasconi; ... et. al J Immunol (2024) 213 (6): 865–875. https://doi.org/10.4049/jimmunol.2400041 Related Content IL-12 De ciency in MRL-Fas lpr Mice Delays Nephritis and Intrarenal IFN-γ Expression, and Diminishes Systemic Pathology J Immunol (April,2003) Ischemia Reperfusion Induces IFN Regulatory Factor 4 in Renal Dendritic Cells, which Suppresses Postischemic In ammation and Prevents Acute Renal Failure J Immunol (August,2010) Immunomodulatory Molecule IRAK-M Balances Macrophage Polarization and Determines Macrophage Responses during Renal Fibrosis J Immunol (August,2017) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/213/6/865/1661067/ji2400041.pdf by guest on 08 February 2025 Renal IL-23 Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with

    Immunocytochemistry:

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL .

    Article Title: GDF11 downregulates FOXP3 in T-cell acute lymphoblastic leukemia-derived cells and associates with restraining aggressiveness
    Article Snippet: Background: Growth differentiation factor 11 (GDF11), a transforming growth factor-beta superfamily member, is a crucial protein involved in many differentiation processes in embryogenesis and morphogenesis, and it has been extensively characterized due to its capacity to target poorly differentiated cells, including transformed or cancer cells.. Aim: In the present work, we aimed to describe the effects on migration, proliferation, and metabolism in the T-cell acute lymphoblastic leukemia-derived cell line Jurkat.. Methods: Based on previous evidence, we analyzed metabolic changes exerted by GDF11 and its relationship with the aggressive phenotype.

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: Cells were incubated for 72 h either without stimulation or with 1 mg/mL immobilized anti-CD3 mAb (eBioscience), 1 mg/mL soluble anti-CD28 mAb (eBioscience), 10 ng/mL IL-2 (PeproTech, London, UK), and 5 ng/mL TGF-b (PeproTech).

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis.
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL92.

    Article Title: Mechanical microscopy of cancer cells: TGF-β induced epithelial to mesenchymal transition corresponds to low intracellular viscosity in cancer cells.
    Article Snippet: Viscosity is an essential parameter that regulates bio-molecular reaction rates of diffusion-driven cellular processes.. Hence, abnormal viscosity levels are often associated with various diseases and malfunctions like cancer.. For this reason, monitoring intracellular viscosity becomes vital.

    Article Title: Mitochondrial reactive oxygen species regulate acetyl-CoA flux between cytokine production and fatty acid synthesis in effector T cells.
    Article Snippet: CD4+CD25T cells were cultured without any polarizing cytokines or antibodies for non-polarizing (ThN) conditions, with 10 mg/ml anti-IL-4 antibody (Peprotech) and 2 ng/ml IL-12 for Th1 differentiation; 20 mg/ml anti-IFN-g antibody (Bio XCell), 2.5 mg/ml anti-IL-12 antibody and 10 ng/ml IL-4 for Th2 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech) and 1 ng/ml TGF-b (Peprotech) for non-pathogenic Th17 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech), 10 ng/ml IL-23 (R&D Systems) and 10 ng/ml IL-1b (R&D Systems) for pathogenic Th17 differentiation and cultured in T cell medium as described above.

    Article Title: Multiplexed PLGA scaffolds with nitric oxide-releasing zinc oxide and melatonin-modulated extracellular vesicles for severe chronic kidney disease.
    Article Snippet: After 24 h of seeding, the cells were damaged with TGF-b (2 ng/ml, 100–21, Peprotech, NJ, USA) for 2 h and PLGA, PME, PMEZ, and PMEZ/mEV (1 108 EVs/mL) scaffolds were treated using trans-well inserts system after 24 h. Cell nucleus and fibronectin were stained using Hoechst (1 lg/ml, 62249, Thermo Fisher Scientific, OH, USA) and fibronectin primary antibody (Abcam, MA, USA) for ICC, and proteins were extracted to compare inhibition activity of scaffolds for fibrosis markers, fibronectin, using Western blot analysis.

    Article Title: Renal IL-23-Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with Kidney Fibrosis.
    Article Snippet: RESEARCH ARTICLE | JULY 29 2024 Renal IL-23–Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal In ammasome Activation with Kidney Fibrosis Teresa M. Frasconi; ... et. al J Immunol (2024) 213 (6): 865–875. https://doi.org/10.4049/jimmunol.2400041 Related Content IL-12 De ciency in MRL-Fas lpr Mice Delays Nephritis and Intrarenal IFN-γ Expression, and Diminishes Systemic Pathology J Immunol (April,2003) Ischemia Reperfusion Induces IFN Regulatory Factor 4 in Renal Dendritic Cells, which Suppresses Postischemic In ammation and Prevents Acute Renal Failure J Immunol (August,2010) Immunomodulatory Molecule IRAK-M Balances Macrophage Polarization and Determines Macrophage Responses during Renal Fibrosis J Immunol (August,2017) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/213/6/865/1661067/ji2400041.pdf by guest on 08 February 2025 Renal IL-23 Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with

    Inhibition:

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL .

    Article Title: GDF11 downregulates FOXP3 in T-cell acute lymphoblastic leukemia-derived cells and associates with restraining aggressiveness
    Article Snippet: Background: Growth differentiation factor 11 (GDF11), a transforming growth factor-beta superfamily member, is a crucial protein involved in many differentiation processes in embryogenesis and morphogenesis, and it has been extensively characterized due to its capacity to target poorly differentiated cells, including transformed or cancer cells.. Aim: In the present work, we aimed to describe the effects on migration, proliferation, and metabolism in the T-cell acute lymphoblastic leukemia-derived cell line Jurkat.. Methods: Based on previous evidence, we analyzed metabolic changes exerted by GDF11 and its relationship with the aggressive phenotype.

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: Cells were incubated for 72 h either without stimulation or with 1 mg/mL immobilized anti-CD3 mAb (eBioscience), 1 mg/mL soluble anti-CD28 mAb (eBioscience), 10 ng/mL IL-2 (PeproTech, London, UK), and 5 ng/mL TGF-b (PeproTech).

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis.
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL92.

    Article Title: Mechanical microscopy of cancer cells: TGF-β induced epithelial to mesenchymal transition corresponds to low intracellular viscosity in cancer cells.
    Article Snippet: Viscosity is an essential parameter that regulates bio-molecular reaction rates of diffusion-driven cellular processes.. Hence, abnormal viscosity levels are often associated with various diseases and malfunctions like cancer.. For this reason, monitoring intracellular viscosity becomes vital.

    Article Title: Mitochondrial reactive oxygen species regulate acetyl-CoA flux between cytokine production and fatty acid synthesis in effector T cells.
    Article Snippet: CD4+CD25T cells were cultured without any polarizing cytokines or antibodies for non-polarizing (ThN) conditions, with 10 mg/ml anti-IL-4 antibody (Peprotech) and 2 ng/ml IL-12 for Th1 differentiation; 20 mg/ml anti-IFN-g antibody (Bio XCell), 2.5 mg/ml anti-IL-12 antibody and 10 ng/ml IL-4 for Th2 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech) and 1 ng/ml TGF-b (Peprotech) for non-pathogenic Th17 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech), 10 ng/ml IL-23 (R&D Systems) and 10 ng/ml IL-1b (R&D Systems) for pathogenic Th17 differentiation and cultured in T cell medium as described above.

    Article Title: Multiplexed PLGA scaffolds with nitric oxide-releasing zinc oxide and melatonin-modulated extracellular vesicles for severe chronic kidney disease.
    Article Snippet: After 24 h of seeding, the cells were damaged with TGF-b (2 ng/ml, 100–21, Peprotech, NJ, USA) for 2 h and PLGA, PME, PMEZ, and PMEZ/mEV (1 108 EVs/mL) scaffolds were treated using trans-well inserts system after 24 h. Cell nucleus and fibronectin were stained using Hoechst (1 lg/ml, 62249, Thermo Fisher Scientific, OH, USA) and fibronectin primary antibody (Abcam, MA, USA) for ICC, and proteins were extracted to compare inhibition activity of scaffolds for fibrosis markers, fibronectin, using Western blot analysis.

    Article Title: Renal IL-23-Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with Kidney Fibrosis.
    Article Snippet: RESEARCH ARTICLE | JULY 29 2024 Renal IL-23–Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal In ammasome Activation with Kidney Fibrosis Teresa M. Frasconi; ... et. al J Immunol (2024) 213 (6): 865–875. https://doi.org/10.4049/jimmunol.2400041 Related Content IL-12 De ciency in MRL-Fas lpr Mice Delays Nephritis and Intrarenal IFN-γ Expression, and Diminishes Systemic Pathology J Immunol (April,2003) Ischemia Reperfusion Induces IFN Regulatory Factor 4 in Renal Dendritic Cells, which Suppresses Postischemic In ammation and Prevents Acute Renal Failure J Immunol (August,2010) Immunomodulatory Molecule IRAK-M Balances Macrophage Polarization and Determines Macrophage Responses during Renal Fibrosis J Immunol (August,2017) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/213/6/865/1661067/ji2400041.pdf by guest on 08 February 2025 Renal IL-23 Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with

    Activity Assay:

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL .

    Article Title: GDF11 downregulates FOXP3 in T-cell acute lymphoblastic leukemia-derived cells and associates with restraining aggressiveness
    Article Snippet: Background: Growth differentiation factor 11 (GDF11), a transforming growth factor-beta superfamily member, is a crucial protein involved in many differentiation processes in embryogenesis and morphogenesis, and it has been extensively characterized due to its capacity to target poorly differentiated cells, including transformed or cancer cells.. Aim: In the present work, we aimed to describe the effects on migration, proliferation, and metabolism in the T-cell acute lymphoblastic leukemia-derived cell line Jurkat.. Methods: Based on previous evidence, we analyzed metabolic changes exerted by GDF11 and its relationship with the aggressive phenotype.

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: Cells were incubated for 72 h either without stimulation or with 1 mg/mL immobilized anti-CD3 mAb (eBioscience), 1 mg/mL soluble anti-CD28 mAb (eBioscience), 10 ng/mL IL-2 (PeproTech, London, UK), and 5 ng/mL TGF-b (PeproTech).

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis.
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL92.

    Article Title: Mechanical microscopy of cancer cells: TGF-β induced epithelial to mesenchymal transition corresponds to low intracellular viscosity in cancer cells.
    Article Snippet: Viscosity is an essential parameter that regulates bio-molecular reaction rates of diffusion-driven cellular processes.. Hence, abnormal viscosity levels are often associated with various diseases and malfunctions like cancer.. For this reason, monitoring intracellular viscosity becomes vital.

    Article Title: Mitochondrial reactive oxygen species regulate acetyl-CoA flux between cytokine production and fatty acid synthesis in effector T cells.
    Article Snippet: CD4+CD25T cells were cultured without any polarizing cytokines or antibodies for non-polarizing (ThN) conditions, with 10 mg/ml anti-IL-4 antibody (Peprotech) and 2 ng/ml IL-12 for Th1 differentiation; 20 mg/ml anti-IFN-g antibody (Bio XCell), 2.5 mg/ml anti-IL-12 antibody and 10 ng/ml IL-4 for Th2 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech) and 1 ng/ml TGF-b (Peprotech) for non-pathogenic Th17 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech), 10 ng/ml IL-23 (R&D Systems) and 10 ng/ml IL-1b (R&D Systems) for pathogenic Th17 differentiation and cultured in T cell medium as described above.

    Article Title: Multiplexed PLGA scaffolds with nitric oxide-releasing zinc oxide and melatonin-modulated extracellular vesicles for severe chronic kidney disease.
    Article Snippet: After 24 h of seeding, the cells were damaged with TGF-b (2 ng/ml, 100–21, Peprotech, NJ, USA) for 2 h and PLGA, PME, PMEZ, and PMEZ/mEV (1 108 EVs/mL) scaffolds were treated using trans-well inserts system after 24 h. Cell nucleus and fibronectin were stained using Hoechst (1 lg/ml, 62249, Thermo Fisher Scientific, OH, USA) and fibronectin primary antibody (Abcam, MA, USA) for ICC, and proteins were extracted to compare inhibition activity of scaffolds for fibrosis markers, fibronectin, using Western blot analysis.

    Article Title: Renal IL-23-Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with Kidney Fibrosis.
    Article Snippet: RESEARCH ARTICLE | JULY 29 2024 Renal IL-23–Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal In ammasome Activation with Kidney Fibrosis Teresa M. Frasconi; ... et. al J Immunol (2024) 213 (6): 865–875. https://doi.org/10.4049/jimmunol.2400041 Related Content IL-12 De ciency in MRL-Fas lpr Mice Delays Nephritis and Intrarenal IFN-γ Expression, and Diminishes Systemic Pathology J Immunol (April,2003) Ischemia Reperfusion Induces IFN Regulatory Factor 4 in Renal Dendritic Cells, which Suppresses Postischemic In ammation and Prevents Acute Renal Failure J Immunol (August,2010) Immunomodulatory Molecule IRAK-M Balances Macrophage Polarization and Determines Macrophage Responses during Renal Fibrosis J Immunol (August,2017) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/213/6/865/1661067/ji2400041.pdf by guest on 08 February 2025 Renal IL-23 Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with

    Western Blot:

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL .

    Article Title: GDF11 downregulates FOXP3 in T-cell acute lymphoblastic leukemia-derived cells and associates with restraining aggressiveness
    Article Snippet: Background: Growth differentiation factor 11 (GDF11), a transforming growth factor-beta superfamily member, is a crucial protein involved in many differentiation processes in embryogenesis and morphogenesis, and it has been extensively characterized due to its capacity to target poorly differentiated cells, including transformed or cancer cells.. Aim: In the present work, we aimed to describe the effects on migration, proliferation, and metabolism in the T-cell acute lymphoblastic leukemia-derived cell line Jurkat.. Methods: Based on previous evidence, we analyzed metabolic changes exerted by GDF11 and its relationship with the aggressive phenotype.

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: Cells were incubated for 72 h either without stimulation or with 1 mg/mL immobilized anti-CD3 mAb (eBioscience), 1 mg/mL soluble anti-CD28 mAb (eBioscience), 10 ng/mL IL-2 (PeproTech, London, UK), and 5 ng/mL TGF-b (PeproTech).

    Article Title: Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis.
    Article Snippet: TGF-b (Peprotech) was added to induce myofibroblast formation at 5 ng/mL92.

    Article Title: Mechanical microscopy of cancer cells: TGF-β induced epithelial to mesenchymal transition corresponds to low intracellular viscosity in cancer cells.
    Article Snippet: Viscosity is an essential parameter that regulates bio-molecular reaction rates of diffusion-driven cellular processes.. Hence, abnormal viscosity levels are often associated with various diseases and malfunctions like cancer.. For this reason, monitoring intracellular viscosity becomes vital.

    Article Title: Mitochondrial reactive oxygen species regulate acetyl-CoA flux between cytokine production and fatty acid synthesis in effector T cells.
    Article Snippet: CD4+CD25T cells were cultured without any polarizing cytokines or antibodies for non-polarizing (ThN) conditions, with 10 mg/ml anti-IL-4 antibody (Peprotech) and 2 ng/ml IL-12 for Th1 differentiation; 20 mg/ml anti-IFN-g antibody (Bio XCell), 2.5 mg/ml anti-IL-12 antibody and 10 ng/ml IL-4 for Th2 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech) and 1 ng/ml TGF-b (Peprotech) for non-pathogenic Th17 differentiation; 10 mg/ml anti-IL-4 antibody, 10 mg/ml anti-IFN-g antibody, 30 ng/ml IL-6 (Peprotech), 10 ng/ml IL-23 (R&D Systems) and 10 ng/ml IL-1b (R&D Systems) for pathogenic Th17 differentiation and cultured in T cell medium as described above.

    Article Title: Multiplexed PLGA scaffolds with nitric oxide-releasing zinc oxide and melatonin-modulated extracellular vesicles for severe chronic kidney disease.
    Article Snippet: After 24 h of seeding, the cells were damaged with TGF-b (2 ng/ml, 100–21, Peprotech, NJ, USA) for 2 h and PLGA, PME, PMEZ, and PMEZ/mEV (1 108 EVs/mL) scaffolds were treated using trans-well inserts system after 24 h. Cell nucleus and fibronectin were stained using Hoechst (1 lg/ml, 62249, Thermo Fisher Scientific, OH, USA) and fibronectin primary antibody (Abcam, MA, USA) for ICC, and proteins were extracted to compare inhibition activity of scaffolds for fibrosis markers, fibronectin, using Western blot analysis.

    Article Title: Renal IL-23-Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with Kidney Fibrosis.
    Article Snippet: RESEARCH ARTICLE | JULY 29 2024 Renal IL-23–Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal In ammasome Activation with Kidney Fibrosis Teresa M. Frasconi; ... et. al J Immunol (2024) 213 (6): 865–875. https://doi.org/10.4049/jimmunol.2400041 Related Content IL-12 De ciency in MRL-Fas lpr Mice Delays Nephritis and Intrarenal IFN-γ Expression, and Diminishes Systemic Pathology J Immunol (April,2003) Ischemia Reperfusion Induces IFN Regulatory Factor 4 in Renal Dendritic Cells, which Suppresses Postischemic In ammation and Prevents Acute Renal Failure J Immunol (August,2010) Immunomodulatory Molecule IRAK-M Balances Macrophage Polarization and Determines Macrophage Responses during Renal Fibrosis J Immunol (August,2017) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/213/6/865/1661067/ji2400041.pdf by guest on 08 February 2025 Renal IL-23 Dependent Type 3 Innate Lymphoid Cells Link Crystal-induced Intrarenal Inflammasome Activation with



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